Dr. Mohammed Al-sbou
|
|
|
|
|
Email |
malsbou@mutah.edu.jo |
|
|
Office Phone |
3191 |
|
|
Academic Rank |
Assistant Professor |
|
|
Faculty |
Medicine |
|
|
Department |
Pharmacology |
|
|
Research of Interests |
Clinical Pharmacology Adverse drug reactions Pharmacogenetics Alkaptonuria |
|
|
Publications and Abstracts |
1. Pharmacogenomics. 2009
Apr;10(4):531-40. TNF, LTA,
HSPA1L and HLA-DR gene polymorphisms in HIV-positive patients with
hypersensitivity to cotrimoxazole.
Alfirevic A, Vilar FJ, Alsbou M, Jawaid A, Thomson W, Ollier WE, Bowman CE, Delrieu O, Park BK, Pirmohamed M.
AIMS: Sulfamethoxazole
in combination with trimethoprim (cotrimoxazole) is used for prophylaxis and treatment of
several opportunistic infections in HIV-infected patients. It is associated
with a high incidence of hypersensitivity reactions, which is thought to have
an immune basis. Genetic polymorphisms in MHC are known to predispose to
hypersensitivity reactions to a structurally diverse group of drugs in
HIV-positive patients. The aim of the study was to determine whether
functional polymorphisms in TNF, LTA, HSPA1L and HLA-DRB1 genes influence the
risk of cotrimoxazole hypersensitivity in
HIV-infected patients. METHODS: We genotyped 136 HIV-positive patients
with (n = 53) and without (n = 83) cotrimoxazole
hypersensitivity using a combination of PCR-based techniques, including
PCR-restriction fragment length polymorphisms, PCR-sequence specific oligonucleotides and real-time PCR. Genotypes and the haplotype frequencies were analyzed using the chi(2) test in the Haploview and
CLUMP programs. Results: No statistically significant difference in SNP or haplotype frequencies were found in HIV-infected sulfamethoxazole hypersensitive patients compared with
controls. CONCLUSION: Our data show that MHC polymorphisms are not
major predisposing factors for cotrimoxazole
hypersensitivity, although we cannot exclude a minor contribution. An
environmental factor (i.e., HIV infection) seems to predominate over any of
the genetic factors so far investigated in increasing the risk of cotrimoxazole hypersensitivity. Publication Date: April 2009 2. Mohammed Alsbou.
Incidence of adverse drug reactions in-patients at Al-karak
governmental hospital in Jordan (in press). 3. Nine cases of Alkaptonuria in one family in Jordan (in press). |